Restriction Enzyme Digestion Exercise – An In-class Activity
نویسندگان
چکیده
منابع مشابه
Detecting differences in 5-methylcytosine using restriction enzyme isoschizomers: an endogenous control for complete digestion.
Growing research effort in recent years has focused on the role of 5-methylcytosine (5-MeC) in gene expression. 5-MeC has been implicated in such phenomena as genomic imprinting, maintenance of heterochromatin, and the direct control of gene expression through proteins which bind methylated DNA (1). As interest in 5-MeC has intensified, several new techniques have been developed to find and stu...
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Present-day DNA technology is partially dependent upon the ability of investigators to cut DNA molecules at specific sites with enzymes called restriction endonucleases or restriction enzymes. The most useful of these enzymes, which occur naturally within bacteria, recognize particular "target" or recognition sequences (restriction sites) within a DNA double helix, and catalyze the cleavage of ...
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introduction corn has been recently used for producing ethanol fuel in the major corn-producing countries such as the us and brazil. recent diversion of corn for biofuel production along with the increased world's demand for this feedstuff has resulted in unprecedented rise in feed cost for poultry worldwide. alternative grains such as wheat and barley can be successfully replaced for corn in p...
متن کاملRestriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae.
Reliable banding techniques are a major necessity for genetic research in oysters. In this study, we carried out the cytogenetic characterization of four oyster species (family Ostreidae) using restriction endonuclease treatments. Chromosomes were treated with three different restriction enzymes, stained with Giemsa, and examined for banding patterns. The following species were studied: Crassos...
متن کاملChromosome restriction enzyme digestion in domestic pig (Sus scrofa) constitutive heterochromatin arrangement.
The bimodal karyotype of pig appears to contain two types of constitutive heterochromatin, reflecting different satellite DNA families: GC-rich heterochromatin located mainly in the centromeric regions of the biarmed chromosomes, and less-GC-rich heterochromatin in the centromeric regions of the one-armed chromosomes. In order to better discriminate this constitutive heterochromatin, we treated...
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ژورنال
عنوان ژورنال: Journal of Microbiology & Biology Education
سال: 2010
ISSN: 1935-7877,1935-7885
DOI: 10.1128/jmbe.v1.i2.129